A live attenuated vaccine prevents replication and transmission of H7N9 virus in mammals.
Identifieur interne : 000599 ( Main/Exploration ); précédent : 000598; suivant : 000600A live attenuated vaccine prevents replication and transmission of H7N9 virus in mammals.
Auteurs : Huihui Kong [République populaire de Chine] ; Qianyi Zhang [République populaire de Chine] ; Chunyang Gu [République populaire de Chine] ; Jianzhong Shi [République populaire de Chine] ; Guohua Deng [République populaire de Chine] ; Shujie Ma [République populaire de Chine] ; Jinxiong Liu [République populaire de Chine] ; Pucheng Chen [République populaire de Chine] ; Yuntao Guan [République populaire de Chine] ; Yongping Jiang [République populaire de Chine] ; Hualan Chen [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Réplication virale, Sous-type H7N9 du virus de la grippe A, Vaccins atténués.
- physiologie : Sous-type H7N9 du virus de la grippe A.
- Animaux.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Vaccines, Attenuated.
- immunology : Influenza A Virus, H7N9 Subtype, Virus Replication.
- physiology : Influenza A Virus, H7N9 Subtype.
- Animals.
Abstract
The continued spread of the newly emerged H7N9 viruses among poultry in China, together with the emergence of drug-resistant variants and the possibility of human-to-human transmission, has spurred attempts to develop an effective vaccine. An MF59-adjuvant H7N9 inactivated vaccine is reported to be well-tolerated and immunogenic in humans; however a study in ferrets indicated that while a single dose of the inactivated H7N9 vaccine reduced disease severity, it did not prevent virus replication and transmission. In this study, we used reverse genetics to produce a cold-adapted, live attenuated H7N9 vaccine (H7N9/AAca) that contains wild-type HA and NA genes from AH/1, and the backbone of the cold-adapted influenza H2N2 A/Ann Arbor/6/60 virus (AAca). H7N9/AAca was attenuated in mice and ferrets, and induced robust neutralizing antibody responses in rhesus mice, ferrets, and guinea pigs immunized once or twice intranasally. The animals immunized twice were completely protected from H7N9 virus challenge. Importantly, the animals vaccinated once were fully protected from transmission when exposed to or in contact with the H7N9 virus-inoculated animals. These results demonstrate that a cold-adapted H7N9 vaccine can prevent H7N9 virus transmission; they provide a compelling argument for further testing of this vaccine in human trials.
DOI: 10.1038/srep11233
PubMed: 26058711
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 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Zhang</name><affiliation wicri:level="1"><nlm:affiliation>1] State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China [2] Department of Inspection Technology Research &National Classical Swine Fever Reference Laboratory, China Institute of Veterinary Drug Control, Beijing 100081, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1] State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China [2] Department of Inspection Technology Research &National Classical Swine Fever Reference Laboratory, China Institute of Veterinary Drug Control, Beijing 100081</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Gu, Chunyang" sort="Gu, Chunyang" uniqKey="Gu C" first="Chunyang" last="Gu">Chunyang Gu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Shi, Jianzhong" sort="Shi, Jianzhong" uniqKey="Shi J" first="Jianzhong" last="Shi">Jianzhong Shi</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Guohua" sort="Deng, Guohua" uniqKey="Deng G" first="Guohua" last="Deng">Guohua Deng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Ma, Shujie" sort="Ma, Shujie" uniqKey="Ma S" first="Shujie" last="Ma">Shujie Ma</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jinxiong" sort="Liu, Jinxiong" uniqKey="Liu J" first="Jinxiong" last="Liu">Jinxiong Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pucheng" sort="Chen, Pucheng" uniqKey="Chen P" first="Pucheng" last="Chen">Pucheng Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Guan, Yuntao" sort="Guan, Yuntao" uniqKey="Guan Y" first="Yuntao" last="Guan">Yuntao Guan</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Yongping" sort="Jiang, Yongping" uniqKey="Jiang Y" first="Yongping" last="Jiang">Yongping Jiang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001</wicri:regionArea><wicri:noRegion>Harbin 150001</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Influenza A Virus, H7N9 Subtype (immunology)</term><term>Influenza A Virus, H7N9 Subtype (physiology)</term><term>Vaccines, Attenuated (immunology)</term><term>Virus Replication (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Réplication virale (immunologie)</term><term>Sous-type H7N9 du virus de la grippe A (immunologie)</term><term>Sous-type H7N9 du virus de la grippe A (physiologie)</term><term>Vaccins atténués (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Réplication virale</term><term>Sous-type H7N9 du virus de la grippe A</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H7N9 Subtype</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H7N9 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H7N9 Subtype</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The continued spread of the newly emerged H7N9 viruses among poultry in China, together with the emergence of drug-resistant variants and the possibility of human-to-human transmission, has spurred attempts to develop an effective vaccine. An MF59-adjuvant H7N9 inactivated vaccine is reported to be well-tolerated and immunogenic in humans; however a study in ferrets indicated that while a single dose of the inactivated H7N9 vaccine reduced disease severity, it did not prevent virus replication and transmission. In this study, we used reverse genetics to produce a cold-adapted, live attenuated H7N9 vaccine (H7N9/AAca) that contains wild-type HA and NA genes from AH/1, and the backbone of the cold-adapted influenza H2N2 A/Ann Arbor/6/60 virus (AAca). H7N9/AAca was attenuated in mice and ferrets, and induced robust neutralizing antibody responses in rhesus mice, ferrets, and guinea pigs immunized once or twice intranasally. The animals immunized twice were completely protected from H7N9 virus challenge. Importantly, the animals vaccinated once were fully protected from transmission when exposed to or in contact with the H7N9 virus-inoculated animals. These results demonstrate that a cold-adapted H7N9 vaccine can prevent H7N9 virus transmission; they provide a compelling argument for further testing of this vaccine in human trials. </div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Kong, Huihui" sort="Kong, Huihui" uniqKey="Kong H" first="Huihui" last="Kong">Huihui Kong</name></noRegion><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name><name sortKey="Chen, Pucheng" sort="Chen, Pucheng" uniqKey="Chen P" first="Pucheng" last="Chen">Pucheng Chen</name><name sortKey="Deng, Guohua" sort="Deng, Guohua" uniqKey="Deng G" first="Guohua" last="Deng">Guohua Deng</name><name sortKey="Gu, Chunyang" sort="Gu, Chunyang" uniqKey="Gu C" first="Chunyang" last="Gu">Chunyang Gu</name><name sortKey="Guan, Yuntao" sort="Guan, Yuntao" uniqKey="Guan Y" first="Yuntao" last="Guan">Yuntao Guan</name><name sortKey="Jiang, Yongping" sort="Jiang, Yongping" uniqKey="Jiang Y" first="Yongping" last="Jiang">Yongping Jiang</name><name sortKey="Liu, Jinxiong" sort="Liu, Jinxiong" uniqKey="Liu J" first="Jinxiong" last="Liu">Jinxiong Liu</name><name sortKey="Ma, Shujie" sort="Ma, Shujie" uniqKey="Ma S" first="Shujie" last="Ma">Shujie Ma</name><name sortKey="Shi, Jianzhong" sort="Shi, Jianzhong" uniqKey="Shi J" first="Jianzhong" last="Shi">Jianzhong Shi</name><name sortKey="Zhang, Qianyi" sort="Zhang, Qianyi" uniqKey="Zhang Q" first="Qianyi" last="Zhang">Qianyi Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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